Genomics Core

Director

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Michael J. Salbaum, PhD

About this Core

The Genomics Core provides high quality data for research. The services of the Genomics Core Facility (GCF) are available to internal Pennington Biomedical researchers and external researchers as well. 

The GCF provides education on optimal use of equipment related to the following services: Sanger and next-generation DNA sequencing; DNA fragment analysis; qualitative and quantitative analysis of DNA, protein, and RNA samples; quantitative PCR; robotics and bioinformatics services. Individual and small group training and consultation services are offered for sequence analysis, real-time PCR, and next-generation sequencing analysis.  

Services are available to any researcher. The goal of the GCF is to provide high quality service to facilitate research. Use of the GCF services are scheduled on a first come, first serve basis. The laboratory is equipped with the following tools: 

  • Applied Biosystems 3130XL: This sixteen capillary genetic analyzer is used to perform DNA sequencing and fragment analysis.  
  • Applied Biosystems 7900HT: Sequence Detection Systems equipped with 96 well, 384 well, and low-density array blocks are available for Quantitative Polymerase Chain Reaction (qPCR).
  • QuantStudio6 and 7Pro: 96 well, 384 well, and low-density array blocks are available for Quantitative Polymerase Chain Reaction (qPCR).   
  • Agilent 2100 Bioanalyzer: Two instruments used for protein, DNA, and RNA analysis and quantitation. 
  • NanoDrop ND-1000 and ND-One Spectrophotometers: These instruments are used for RNA and DNA quantification. 
  • 10x Genomics Chromium X and CytAssist: Instrumentation for Single-Cell Sequencing and Spatial Transcriptomics  
  • Qubit Fluorometer: This instrument is used for RNA and DNA quantification.. 
  • Illumina MiSeq and NextSeq2000: Next Generation Sequencers to address Metagenomics, Gene Expresion, ChIP-Seq, RNA-Seq, Small RNA Sequencing Assays. Many other applications are supported as well. 
  • Eppendorf EPMotion 5075TMX: Pipetting robot available for robotic liquid handling. These instruments facilitate high-throughput pipetting of 384 well format plates and library construction for NGS. Can be programmed for large pipetting projects. 

For more information about the CGF lab and to submit a sequencing request, visit: gcf.pbrc.edu