Bioinformatics and Molecular Mechanisms Core
The Bioinformatics and Molecular Mechanisms Core (BMMC) delivers critical molecular, cellular and data analytic technologies to investigate how nutritional and metabolic factors influence disease mechanisms. Positioned as a translational bridge between basic and clinical studies, the BMMC enables integrated research strategies that support both discovery science and the development of interventions to treat or prevent metabolic disease. The BMMC fulfills its mission through its three Subcores - Genomics, Bioimaging, and Bioinformatics - providing comprehensive support for molecular and cellular investigations and creating a seamless research pipeline from consultation to publication.
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General Inquiries |
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Director (Dr. Sujoy Ghosh) P: 225-763-2837
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Assoc. Director (Dr. David Burk) P: 225-763-2779 |
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Subcore Inquiries |
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Genomics Dr. Michael Salbaum P: 225-763-2782 |
Bioimaging Dr. David Burk P: 225-763-2779 |
Bioinformatics Dr. Sujoy Ghosh P: 225-763-2837 |
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Genomics Subcore
Dr. Michael Salbaum
The Genomics Subcore provides expertise and services for:
- Consultation on all aspects of genomics experiments
- Nucleic Acid measurement and Quality Control
- Next Generation Sequencing services (bulk RNA sequencing, single-cell RNA sequencing, ATAC-Seq, ChIP-Seq, metagenomics sequencing, spatial transcriptomics)
- Sanger sequencing
- Polymerase Chain Reaction (Quantitative and Digital Droplet PCR)
- Manuscript and grant writing support
Bioimaging Subcore
Dr. David Burk
The Bioimaging Subcore provides expertise and services for:
- Consultation on all aspects of bioimaging experiments
- Histology services
- Imaging services
- Light sheet fluorescence microscopy
- Image analysis
- Virtual reality visualization and 3D rendering
- Manuscript and grant writing support
Bioinformatics Subcore
Dr. Sujoy Ghosh
The Bioinformatics Subcore will provide expertise and services for:
- Consultation on all aspects of bioinformatics data analysis
- Microarray and pre-NGS data analysis
- Next-generation sequencing data analysis (bulk and single-cell RNA-Seq, ChIP-Seq, ATAC-Seq, metagenomics, spatial transcriptomics)
- Other omics (proteomics, metabolomics, etc.)
- Genome-wide association studies (GWAS)
- Machine learning/AI applications
- Manuscript and grant writing support
Genomics Services
The Genomics Subcore provides services and technical expertise for the isolation and analysis of chromatin and RNA from both animal and human samples. Key services offered:
- Consultation on experimental design, technology selection and data analysis
- Next-generation sequencing
- Gene expression - RNA-Seq: 3’ -expression tag sequencing
- Gene expression - single cell RNA sequencing: 10xGenomics
- Chromatin – ChIPSeq & ATAC-Seq
- Microbiota: 16S metagenomics sequencing
- Sanger sequencing
- Gene Expression: Quantitative PCR; AB7900; robotic pipetting of 96- and 384-well plates
- Digital Droplet PCR
- Nucleic Acid measurement and Quality Control: Agilent Bioanalyzer, Nanodrop, Qbit
- Manuscript and grant writing support
To learn more about the Genomics Subcore, click here
Bioimaging Services
The Bioimaging Subcore provides the necessary infrastructure and expertise for examining cellular and tissue-level phenotypes through histological, analytical FACS, and microscopy techniques. Key services offered:
- Consultation on experimental design, technology selection, and data analysis
- Histology: Tissue processing, embedding, sectioning, staining
- Imaging: Leica SP5, DM6000, Lionheart, DMI6000
- Live cell and multi-well plate imaging.
- Tissue clearing – i.e., iDISCO, CUBIC, etc.
- Light sheet fluorescence microscopy of cleared tissue
- Immunohistological staining
- Whole slide scanning: NanoZoomer, Axioscan 7
- Image analysis: Imaris, FIJI, CellProfiler, Visiopharm
- Quantitative histology: adipocyte size; WAT/BAT transitions; Pancreas/Islet biology
- Virtual reality visualization and rendering of 3D datasets
- Manuscript and grant writing support
To learn more about the Bioimaging Subcore, click here
Bioinformatics Services
Data generated from the Genomics and Bioimaging Subcores, as well as externally derived datasets are evaluated within the Bioinformatics Subcore. Key services offered:
- Consultation on experimental design, technology selection, data analysis and interpretation
- Bulk RNA seq data analysis
- Single cell RNA seq data analysis
- Single cell multi-omics data analysis
- Chip-Seq data analysis
- ATAC-Seq data analysis
- DNA methylation data analysis
- GWAS analysis
- Expression quantitative trait loci analysis
- Proteomics data analysis
- Metabolomics data analysis
- Pathway enrichment analysis
- Network analysis
- Machine learning and artificial intelligence data analysis
- Manuscript and grant writing support
To learn more about the Bioinformatics Subcore, click here
As a reminder, please acknowledge support from the Pennington/Louisiana Nutrition Obesity Research Center – Bioinformatics and Molecular Mechanisms Core in your Publications by citing NIDDK Grant Number P30DK072476. Example: This work was partially supported by a NORC Center Grant # P30DK072476 entitled “Nutrition and Metabolic Health Through the Lifespan” sponsored by NIDDK.
