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Proteomics and Metabolomics Core

Director

Mission

The mission of this core is to provide scientific and technical expertise and resources to enable researchers to apply proteomics and metabolomics for biomedical and biological studies.

ABOUT THIS CORE

The Pennington Biomedical Research Center has developed a state-of-the-art high throughput proteomics and metabolomics core facility. This facility provides a variety of technical services for targeted and global proteomics and metabolomics studies. The facility supports activities ranging from detailed characterization of a single peptide, protein or metabolite to global, unbiased analysis of thousands of proteins or metabolites in one experiment. The proteomics capabilities of the facility include: identification and quantification of proteins within cells, secreted media, subcellular fractions, tissue or biological fluids; protein modification analysis; characterization of protein complexes and identification of protein interaction partners; and protein structure determination. The metabolomics capabilities of the facility include: identification, quantification and characterization of small molecules such as lipids, fatty acids and amino acids present in tissues and biological fluids.

The facility utilizes powerful separation technologies such as two-dimensional gel electrophoresis and multi-dimensional liquid chromatography in conjunction with mass spectrometric detection techniques to analyze proteins and metabolites. The facility is equipped with four ESI–tandem mass spectrometers, HPLC and UPLC systems, a 3-color fluorescence scanner, robotic workstations for excising gel bands and protein digestion and a MALDI-TOF mass spectrometer. The facility also maintains a variety of software packages to provide bioinformatics support for proteomics and metabolomics applications.

For more information, visit our core website: http://labs.pbrc.edu/proteomics